The characteristic and anatomic distribution of PD-1 protein in uteri from WT mice was detected by immunohistochemistry and immunofluorescent double staining. Uteri from 2-year old PD-1-/- and WT mice were selected to detect the expression of PD-1 by Immunohistochemistry. (A) Uteri from 2-year old PD-1-/- mice showed no positive staining; However, uteri from 2-year old WT mice showed PD-1-positive cells were observed in glandular/luminal epithelium (B) and filtrating the stroma (C), ▲indicates the glandular epithelium; *indicates the luminal cavity and the arrows indicate positive cells infiltrating the stroma. (D) immunofluorescent double staining showed that PD-1 was expressed on CD68+ macrophages, CD3+ T cells, CD16+ monocytes, CD56+ NK cells and CK-18+ epithelial cells, but PD-1 expression was absence on CD31+ endothelial cells. The blue color indicates nuclear staining with 4′,6-diamidino-2-phenylindole (DAPI), arrows indicate positive cells. Scale bar = 20 μm. N = 4 of each group.
Guo et al. Diagnostic Pathology 2014 9:97 doi:10.1186/1746-1596-9-97