Enhanced PCNA positive cells in PD-1-/- uteri was measured by immunofluorescent staining. Uteri from 2-year old PD-1-/- and WT mice were selected to detect the expression of PCNA by immunofluorescent staining (A). LE: luminal epithelium; S: stroma. The blue color indicates nuclear staining with 4′,6-diamidino-2-phenylindole (DAPI). Scale bar = 20 μm. N = 4 of each group. (B) The PCNA-positive nuclei in uterine tissues between PD-1-/- and WT mice were counted and compared. *p < 0.05.
Guo et al. Diagnostic Pathology 2014 9:97 doi:10.1186/1746-1596-9-97