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Open Access Research

Prognostic significance of HLA EMR8-5 immunohistochemically analyzed expression in osteosarcoma

Ola H Nada1*, Naglaa S Ahmed2 and Hoda H Abou Gabal3

Author Affiliations

1 Department of pathology, faculty of Medicine, Ain Shams university, Cairo, Egypt

2 Department of pathology, faculty of Medicine, Ain Shams university, Cairo, Egypt

3 Department of pathology, faculty of Medicine, Ain Shams university, Cairo, Egypt

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Diagnostic Pathology 2014, 9:72  doi:10.1186/1746-1596-9-72

Published: 25 March 2014

Abstract

Background

Defects in Human Leukocyte Antigen (HLA) class I antigen expression and/or function in tumor cells have been extensively investigated, because of their potential role in the escape of tumor cells from T cell recognition and destruction. The researchers evaluated HLA class I expression in tumor tissue as a prognostic factor in osteosarcoma patients and as a predictor of their survival. This retrospective cohort study was conducted at the pathology laboratory of Ain Shams University Hospital, and Ain Shams University Specialized Hospital during the period between January 2009 and January 2012.

Methods

The researchers investigated HLA class I expression in primary osteosarcoma by immunohistochemistry using EMR8-5 mAbs. Furthermore, researchers evaluated the correlation between HLA class I expression and the clinicopathological status and outcome in formalin fixed paraffin embedded tissues from thirty six (36) patients with osteosarcoma.

Results

A high expression of HLA class I was detected in 18 (50) % of tumor samples examined; while tumors with low or negative expression represented 9 (25%) cases each. Data indicate that the overall survival rate of patients with tumors highly expressing HLA class I was significantly higher than those with low or negative expression.

Conclusion

Down-regulation of class I antigen expression is associated with features of aggressive disease and a poorer prognosis. Therefore, it is imperative to identify HLA as a prognostic factor at the time of diagnosis to detect chemotherapy-resistant tumors and to generate a modified treatment regimen.

Virtual slides

The virtual slides for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1159334857109547 webcite.

Keywords:
Osteosarcoma; Human leukocyte antigen; Prognostic factors; Immunohistochemistry