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Open Access Research

Identification alpha-2-HS-glycoprotein precursor and tubulin beta chain as serology diagnosis biomarker of colorectal cancer

Nai-Jun Fan12, Rui Kang1, Xue-Yan Ge1, Ming Li3, Yan Liu4, Hong-Mei Chen5 and Chun-Fang Gao1*

Author Affiliations

1 Institute of Anal-colorectal Surgery, No.150 Central Hospital of PLA, Luoyang, RP China

2 The General Hospital of Jinan Military Region, Jinan, China

3 Respiration Department, No.150 Central Hospital of PLA, Luoyang, RP China

4 Neuro- Surgery Department, No.150 Central Hospital of PLA, Luoyang, RP China

5 Clinical Laboratory, The First People’s Hospital of the City of Luoyang, Luoyang, RP China

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Diagnostic Pathology 2014, 9:53  doi:10.1186/1746-1596-9-53

Published: 12 March 2014

Abstract

Background

Colorectal cancer (CRC) remains a major worldwide cause of cancer-related morbidity and mortality largely due to the insidious onset of the disease. The current clinical procedures utilized for disease diagnosis are invasive, unpleasant, and inconvenient. Hence, the need for simple blood tests that could be used for the early detection is crucial for its ultimate control and prevention.

Methods

The present work is a case–control study focused on proteomic analysis of serum of healthy volunteers and CRC patients by the ClinProt profiling technology based on mass spectrometry. This approach allowed to identifying a pattern of proteins/peptides able to differentiate the studied populations. Moreover, some of peptides differentially expressed in the serum of patients as compared to healthy volunteers were identified by LTQ Orbitrap XL.

Results

A Quick Classifier Algorithm was used to construct the peptidome patterns (m/z 1208, 1467, 1505, 1618, 1656 and 4215) for the identification of CRC from healthy volunteers with accuracy close to 100% (>CEA, P < 0.05). Peaks at m/z 1505 and 1618 were identified as alpha-2-HS-glycoprotein precursor and tubulin beta chain, respectively.

Conclusions

Alpha-2-HS-glycoprotein precursor and tubulin beta chain could be involved in the pathogenesis of CRC and perform as potential serology diagnosis biomarker.

Virtual slides

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/4796578761089186 webcite.

Keywords:
Colorectal neoplasms; Diagnosis; Biological markers; Proteomics; Alpha-2-HS-glycoprotein; Tubulin beta chain