Table 3

Helicobacteraceae DNA was detected by nested PCR in colonic biopsies
Primera Sequence (5' - 3')b Product size (bp) PCR conditions Target sequence Referencesc
H276f CTA TGA CGG GTA TCC GGC 374 94°c for 15 min and 30 s,52°c for 30 s,72°c for 30 s, 35 cycles,72°c for 7 min Helicobacter spp.16S rRNA Riley et al. [17]
H676r ATT CCA CCT ACC TCT CCC A
p17f ATG GAA CAG ATA AAG ATT TTA AAG CAA CTT CAG 435 94°c for 15 min and 30 s,55°c for 30 s,72°c for 30 s, 35 cycles,72°c for 7 min H. bilis specifict protein Feng et al. [21]
p17r CTA TGC AAG TTG TGC GTT AAG CAT
Fe1F TTT GGT GCT CAC TAA CGC CCT C 434 (438) 94°c for 15 min and 30 s,52°c for 30 s,72°c for 30 s, 35 cycles,72°c for 7 min H. felis urease gene Baele et al. [19]
Fe3R TTC AAT CTG ATC GCG TAA AG
Bi1F AAC CAA YAG CCC CAG CAG CC 373 94°c for 15 min and 30 s,54°c for 30 s,72°c for 30 s, 35 cycles,72°c for 7 min H. bizzozeronii urease gene Baele et al. [19]
Bi2R TGG TTT TAA GGT TCC AGC GC
HSALF CAT TTT CAA AGA GGG CTT GC 537 94°c for 15 min and 30 s,51°c for 30 s,72°c for 30 s, 35 cycles,72°c for 7 min H. salomonis HSP60 Mikkonen et al. [20]
HSALR GCA CAC CCC TCA GTT TGT TT
HP-FOR TTA TCG GTA AAG ACA CCA GAA A 132 94°c for 15 min and 30 s,49°c for 30 s,72°c for 30 s, 35 cycles,72°c for 7 min H. pylori urease C gene He et al. [22]
HP-REV ATC ACA GCG CAT GTC TTC
canis3Fd TAA GCG CGG TAT GGA TAA GG 254 94°c for 15 min and 30 s,52°c for 30 s,72°c for 30 s, 35 cycles,72°c for 7 min H. canis HSP60 EU233451
canis4Rd TTA AGT AGC CGC GGT CAA AC

a:Different Types of Primers, b:Sequence of Helicobacteraceae DNA, c:References studied for all the selected procedures, d:comparison between numerical continuous of the primer in the helicobacter canis.

Abdi et al.

Abdi et al. Diagnostic Pathology 2014 9:50   doi:10.1186/1746-1596-9-50

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