Email updates

Keep up to date with the latest news and content from Diagnostic Pathology and BioMed Central.

Open Access Research

Dry eye management in a Sjögren’s syndrome mouse model by inhibition of p38-MAPK pathway

Xiaoyun Ma1, Jun Zou2*, Linping He1 and Yun Zhang3*

Author Affiliations

1 Department of Ophthalmology, Guanghua Integrative Medicine Hospital, Shanghai, Changning District, China

2 Department of Ophthalmology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, China

3 Institute of Arthritis Research, Shanghai Academy of Chinese Medical Sciences, Shanghai, China

For all author emails, please log on.

Diagnostic Pathology 2014, 9:5  doi:10.1186/1746-1596-9-5

Published: 20 January 2014

Abstract

Background

To investigate the therapeutic effect of p38-MAPK inhibitor, SB203580, on dry eye in a mouse model of Sjögren’s syndrome (MRL/lpr mice).

Methods

18 female BALB/c mice and 44 female MRL/lpr mice were included. Mice were randomly assigned to the control or treatment group. The expression of phospho-p38 MAPK in lacrimal glands of BALB/c mice was determined by Western blot analysis following IL-1β treatment at various time points. Different doses of SB203580 were injected into lacrimal glands of MRL/lpr mice and phenol red thread test was performed seven days post-injection. Moreover, the levels of acetylcholine and norepinephrine expression in lacrimal glands of MRL/lpr mice were measured using spectrofluoremetric assay and the histopathology of lacrimal glands was also evaluated.

Results

The expression of p-p38 MAPK in lacrimal glands of BALB/c mice gradually increased following incubation with IL-1β ex vivo. Injection of SB203580 into lacrimal glands significantly improved the results of phenol red thread test in MRL/lpr mice. In addition, the secretions of acetylcholine and norepinephrine increased significantly compared to the control group. Less lymphocytes infiltration was observed in pathological section of lacrimal glands following SB203580 injection.

Conclusions

Our results indicate that the activation of p38-MAPK pathway plays an important role in dry eye of a Sjögren’s syndrome mouse model. Inhibition of p38-MAPK pathway by SB203580 might have potential therapeutic effect on Sjögren’s syndrome associated dry eye.

Virtual slides

The virtual slides for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1256849631103092 webcite.

Keywords:
Sjögren’s syndrome; Dry eye; Interleukin-1β; p38-MAPK pathway