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Open Access Research

Diagnosis of Trichomonous vaginalis by microscopy, latex agglutination, diamond’s media, and PCR in symptomatic women, Khartoum, Sudan

Amir M Saleh1, Hamid S Abdalla1, Abdelsalam B Satti1, Suad M Babiker1, Gasim I Gasim2 and Ishag Adam3*

Author Affiliations

1 Faculty of Medical Laboratory Sciences, University of Omdurman, Omdurman, Sudan

2 College of Medicine, Qassim University, Buraydah, Qassim, Saudi Arabia

3 Faculty of Medicine, University of Khartoum, Khartoum, Sudan

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Diagnostic Pathology 2014, 9:49  doi:10.1186/1746-1596-9-49

Published: 6 March 2014

Abstract

Background

Trichomoniasis is the most common sexually transmitted disease. However, limited data are available on an effective technique for the diagnosis of Trichomonas vaginalis.

Methods

A cross-sectional study was conducted to evaluate the accuracy of wet mount microscopy, latex agglutination, Diamond’s media, and polymerase chain reaction (PCR) for detection of T. vaginalis among symptomatic women who attended the gynecological clinic at Khartoum, Sudan.

Results

Of the 297 women studied, 252 (84.8%) were positive for T. vaginalis by wet mount microscopy, 257 (86.5%) by latex agglutination, 253 (85.2%) by Diamond’s media, and 253 (85.2%) by PCR. The sensitivity and specificity of wet mount microscopy were 99.2% and 97.7%, respectively, compared with PCR. The sensitivity and specificity of latex agglutination and Diamond’s media were 99.6% and 88.6%, and 100.0% and 86.4%, respectively, compared with PCR.

Conclusions

In this study, wet mount microscopy, latex agglutination, and Diamond’s media were found to be highly sensitive and specific. However, the availability and cost effectiveness might limit the use of Diamond’s media and PCR in routine practice.

Virtual slides

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/7859723851211496. webcite

Keywords:
Latex agglutination; Diamond’s media; PCR; Trichomonas vaginalis; Sudan