Western blot analysis for p-mTOR, TSC1 and p-tuberin (Thr1462) in NSCLC and SCLC cell lines. In GLC-8, MBA 9812 and HCC-827 cells, we found an inverse correlation between hamartin and p-mTOR concentrations. Higher TSC1 levels were associated with low levels of p-mTOR and, vice versa a substantial expression of p-mTOR was obtained in association with decreased levels of TSC1. There was no obvious correlation between p-tuberin levels with those of hamartin or p-mTOR. NCI-H460: large cell cancer of the lung, MBA 9812: SCC, HCC-827: AC harboring an acquired mutation in the EGFR tyrosine kinase domain (E746 - A750 deletion), GLC-2 and GLC-8: SCLC.
Fuchs et al. Diagnostic Pathology 2014 9:48 doi:10.1186/1746-1596-9-48