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Upregulation of microRNA-25 associates with prognosis in hepatocellular carcinoma

Zhong-xue Su1*, Juan Zhao2, Zhong-hou Rong1, Wen-mao Geng1, Ya-guang Wu1 and Cheng-kun Qin1

Author Affiliations

1 Department of Hepatobiliary Surgery, ShanDong Provincial Hospital affiliated to ShanDong University, 324 JingWu Road, Jinan, ShanDong Province 250021, China

2 Department of Hematology, ShanDong Provincial Hospital affiliated to ShanDong University, Jinan, ShanDong Province 250021, China

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Diagnostic Pathology 2014, 9:47  doi:10.1186/1746-1596-9-47

Published: 4 March 2014



Accumulating evidence has shown that up-regulation of microRNA-25(miR-25) is associated with the prognosis of several types of human malignant solid tumors. However, whether miR-25 expression has influence on the prognosis of hepatocellular carcinoma (HCC) is still unknown.


The differentially expressed amount of the miR-25 was validated in triplicate by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Survival rate was analyzed by log-rank test, and survival curves were plotted according to Kaplan–Meier. Multivariate analysis of the prognostic factors was performed with Cox regression model.


The expression of miR-25 was significantly upregulated in HCC tissues when compared with adjacent normal tissues (p<0.0001). Patients who had high miR-25 expression had a shorter overall survival than patients who had low miR-25 expression (median overall survival, 31.0 months versus 42.9 months, p=0.0192). The multivariate Cox regression analysis indicated that miR-25 expression (HR=2.179; p=0.001), TNM stage (HR=1.782; p=0.014), and vein invasion (HR=1.624; p=0.020) were independent prognostic factors for overall survival.


Our data suggests that the overexpression of miR-25 in HCC tissues is of predictive value on poor prognosis.

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