Table 1

PTEN primers used for single-strand conformation polymorphism (SSCP) analysis
Exon Primer sequences Annealing temp. (°C) Amplicon (bp)
1 F1. 5’-TCCTCCTTTTTCTTCAGCCAC-3’ 56 147
R1. 5’-GAAAGGTAAAGAGGAGCAGCC-3’
2 F2. 5’-TGCATATTTCAGATATTTCTTTCCTT-3’ 57 155
R2. 5’-TTTGAAATAGAAAATCAAAGCATTC-3’
3 F3. 5’-TGTTAATGGTGGCTTTTTG-3’ 56 114
R3. 5’-GCAAGCATACAAATAAGAAAAC-3’
4 F4. 5’-TTCCTAAGTGCAAAAGATAAC-3’ 56 147
R4. 5’-TACAGTCTATCGGGTTTAAGT-3’
5 F5. 5’-TTTTTTTTTCTTATTCTGAGGTTATC-3’ 51 312
R5. 5’-GAAGAGGAAAGGAAAAACATC-3’
6 F6. 5’-AGTGAAATAACTATAATGGAACA-3’ 54 231
R6. 5’-GAAGGATGAGAATTTCAAGC-3’
7 F7. 5’-ATCGTTTTTGACAGTTTG-3’ 55 262
R7. 5’-TCCCAATGAAAGTAAAGTAGA-3’
8 F8. 5’-AGGTGACAGATTTTCTTTTTTA-3’ 52 394
R8. 5’-TAGCTGTACTCCTAGAATTA-3’
9 F9. 5’-CTTTCTCTAGGTFAAGCTGTACTT-3’ 55 231
R9. 5’-TTCATGGTGTTTTATCCCTCTTGA-3’

Li et al.

Li et al. Diagnostic Pathology 2014 9:17   doi:10.1186/1746-1596-9-17

Open Data