Assessment of IgG antibodies to Pseudomonas aeruginosa in patients with cystic fibrosis by an enzyme-linked immunosorbent assay (ELISA)
1 Department of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas, Rua Alexander Fleming 105, Block FCM 12, 2nd floor, Campinas, Postal code: 13083-881, SP, Brazil
2 Department of Pediatrics, Faculty of Medical Sciences, State University of Campinas, Campinas SP, Brazil
Diagnostic Pathology 2014, 9:158 doi:10.1186/s13000-014-0158-zPublished: 22 August 2014
The usefulness of serological tests for detection of P. aeruginosa pulmonary infection in cystic fibrosis (CF) is controversial. Here, we assessed the value of detecting anti-P. aeruginosa IgG by a quantitative enzyme-linked immunosorbent assay (ELISA) for identification of P. aeruginosa infection in patients with cystic fibrosis.
Serum concentrations of anti-P. aeruginosa IgG were assessed in 117 CF patients classified according to their P. aeruginosa colonization/infection status (never colonized; free of infection; intermittently colonized and chronically infected) and in 53 healthy subjects by the ELISA test standardized with the St-Ag:1–17 antigen.
The rate of IgG seropositivity and the median of IgG concentrations of this antibody in patients chronically infected were significantly higher than those found in the other CF groups and in the healthy control group.
Detection of anti-P. aeruginosa IgG can be an useful tool for identification of P. aeruginosa chronic infection in patients with CF.
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_158 webcite