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Open Access Research

Assessment of IgG antibodies to Pseudomonas aeruginosa in patients with cystic fibrosis by an enzyme-linked immunosorbent assay (ELISA)

Renan Marrichi Mauch1, Cláudio Lúcio Rossi1, José Dirceu Ribeiro2, Antônio Fernando Ribeiro2, Marcos Tadeu Nolasco da Silva2 and Carlos Emílio Levy1*

Author Affiliations

1 Department of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas, Rua Alexander Fleming 105, Block FCM 12, 2nd floor, Campinas, Postal code: 13083-881, SP, Brazil

2 Department of Pediatrics, Faculty of Medical Sciences, State University of Campinas, Campinas SP, Brazil

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Diagnostic Pathology 2014, 9:158  doi:10.1186/s13000-014-0158-z

Published: 22 August 2014

Abstract (provisional)

BackgroundThe usefulness of serologic tests for detection of P. aeruginosa pulmonary infection in cystic fibrosis (CF) is controversial. Here, we assessed the value of detecting anti-P. aeruginosa IgG by a quantitative enzyme-linked immunosorbent assay (ELISA) for identification of P. aeruginosa infection in patients with cystic fibrosis.MethodsSerum concentrations of anti-P. aeruginosa IgG were assessed in 117 CF patients classified according to their P. aeruginosa colonization/infection status (never colonized; free of infection; intermittently colonized and chronically infected) and in 53 healthy subjects by the ELISA test standardized with the St-Ag:1?17 antigen.ResultsThe rate of IgG seropositivity and the median of IgG concentrations of this antibody in patients chronically infected were significantly higher than those found in the other CF groups and in healthy control group.ConclusionDetection of anti-P. aeruginosa IgG can be an useful tool for identification of P. aeruginosa chronic infection in patients with CF.Virtual SlidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_158

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