Accumulation of connective tissue growth factor+ cells during the early phase of rat traumatic brain injury
1 Institute of Immunology, Third Military Medical University, 30 Gaotanyan Main Street, Chongqing 400038, People’s Republic of China
2 Institute of Brain Research, University of Tuebingen, Tuebingen, Germany
Diagnostic Pathology 2014, 9:141 doi:10.1186/1746-1596-9-141Published: 10 July 2014
Glial scar formation is a common histopathological feature of traumatic brain injury (TBI). Astrogliosis and expression of transforming growth factor beta (TGF-β) are key components of scar formation and blood-brain barrier modulation. Connective tissue growth factor (CTGF) is considered a cytokine mediating the effects of TGF-β.
Here, we studied the CTGF expression in an open-skull weight-drop-induced TBI, with a focus on the early phase, most amenable to therapy.
In normal rat brains of our study, CTGF+ cells were rarely observed. Significant parenchymal accumulation of CTGF+ non-neuron cells was observed 72 h post-TBI and increased continuously during the investigating time. We also observed that the accumulated CTGF+ non-neuron cells were mainly distributed in the perilesional areas and showed activated astrocyte phenotypes with typical stellate morphologic characteristics.
Our observations demonstrated the time-dependent and lesion-associated accumulation of cellular CTGF expression in TBI, suggesting a pathological role of CTGF in TBI.
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