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Open Access Research

Silencing BMP-2 expression inhibits A549 and H460 cell proliferation and migration

Heying Chu1, Hailan Luo2, Huaqi Wang1, Xiaonan Chen3, Ping Li1, Yong Bai1, Furui Zhang1, Ruirui Cheng1, Shanshan Chen1, Yuanyuan Wang3, Guoqiang Zhao3* and Guojun Zhang1*

Author Affiliations

1 Department of Respiratory Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China

2 Department of Medical Biology, Luohe Medical College, Luohe, Henan 462002, China

3 College of Basic Medical Sciences, Zhengzhou University, No.100 Kexue Road, Zhengzhou 450001, China

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Diagnostic Pathology 2014, 9:123  doi:10.1186/1746-1596-9-123

Published: 19 June 2014

Abstract

Background

Bone morphogenetic protein 2 (BMP-2) is a member of the TGF-β superfamily that is closely correlated with many malignancies, particularly lung cancer. However, the effects of silenced BMP-2 on lung cancer cell proliferation and migration are not clear.

Methods

Using quantitative real-time RT-PCR, BMP-2 mRNA expression was detected in 61 non-small cell lung cancer (NSCLC) samples. Survival curves were generated using follow-up data. Relationships between clinical or pathological characteristics and prognosis were analyzed. Cell viability assays and transwell migration assays were used to evaluate the effects of BMP-2 silencing on cell proliferation and migration of A549 and H460 cells.

Results

BMP-2 mRNA expression was higher in NSCLC tissues compared to matched adjacent normal tissues (P < 0.01). High BMP-2 expression levels were significantly associated with the occurrence of lymph node metastases and tumor stage (P < 0.05). There were significant differences in survival curves between groups with metastatic lymph nodes and non-metastatic lymph nodes, as well as between groups with low BMP-2 expression and groups with high BMP-2 expression. In addition, we observed decreased proliferation and migration rates of the NSCLC-derived cell lines A549 and H460 that were transfected with siBMP-2 (P < 0.05).

Conclusion

BMP-2 mRNA is overexpressed in NSCLC samples and is a risk factor for survival in patients with NSCLC. BMP-2 silencing can significantly inhibit A549 and H460 cell proliferation and migration.

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/4263254471298866 webcite

Keywords:
Non-small cell lung cancer (NSCLC); BMP-2; siRNA; Proliferation; Migration