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Cyto-histopathological and outcome features of the prepuce squamous cell carcinoma of a mixed breed dog

Javad Yaghoobi Yeganeh Manesh1, Radmehr Shafiee2, Ali Mohammad Bahrami3, Mehdi Pourzaer4, Maryam Pourzaer5, Behnam Pedram6, Javad Javanbakht7*, Aram Mokarizadeh8* and Farshid Khadivar2

Author Affiliations

1 Gradute of Islamic Azad University of Shahrekord, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran

2 Graduate, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran

3 Faculty of Para Veterinary Medicine, Ilam University, Ilam, Iran

4 Graduate Student, Faculty of Veterinary Science, Karaj Branch, Islamic Azad University, Alborz, Iran

5 Department of Biology, Faculty of Basic Science, Damghan Branch, Islamic Azad University, Damghan, Iran

6 Department of Pathobiology, Susangerd Branch, Islamic Azad University, Susangerd, Iran

7 Department of Pathology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran

8 Department of Immunology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran

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Diagnostic Pathology 2014, 9:110  doi:10.1186/1746-1596-9-110

Published: 6 June 2014



Squamous cell carcinomas (SCCs) are uncommon, high-grade tumors, predominantly composed of round cells in the prepuce. The aim of this study is to better define the clinicopathologic features of this neoplasm.

Case report

We conducted cyto-histopathologic analysis on the manifestations of the prepuce SCC by H & E staining in a terrier mix dog. Grossly, tumor was large, multiple erythematous patch, and ulcerated masses frequently affecting the prepuce and deeply invading to distal prepuce out from the ventro-lateral of penis and the tumor covered by a necrotic discharge. Cytological evaluation of fine-needle aspirates from the cutaneous mass from the prepuce comprised of round nuclei, coarse chromatin pattern, distinct nucleoli and nuclear pleomorphism. Furthermore, the neoplastic cells were pleomorphic, round to caudate in shape, exhibiting prominent anisokaryosis and anisocytosis with rare mitotic features. Microscopically, the lesions were predominantly composed of atypical round cells disposed in interlacing fascicles. Frequent findings include keratin formation, horn pearls, mitoses and cellular atypia. The cells showed distinct borders, ranged from polygonal to round or elongate and had moderate amounts of eosinophilic cytoplasm.


The histopathologic features coupled with the cytopathology findings led to a diagnosis of squamous cell carcinoma. To the authors’ knowledge, this is the first time that multiple erythematous plaques have undergone malignant transformation in a terrier mix dog.

The virtual slide(s) for this article can be found here: webcite

Histopathology; Cytology; Dog; Prepuce; Tumour