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This article is part of the supplement: Proceedings of the 11th European Congress on Telepathology and 5th International Congress on Virtual Microscopy

Open Access Proceedings

Automated segmentation of blood cells in Giemsa stained digitized thin blood films

Margarita Walliander1*, Riku Turkki12, Nina Linder1, Mikael Lundin1, Juho Konsti1, Ville Ojansivu12, Taru Meri3, Ville Holmberg3 and Johan Lundin1

Author Affiliations

1 Institute for Molecular Medicine Finland (FiMM), Finland

2 Center for Machine Vision Research, Department of Computer Science and Engineering, University of Oulu, Finland

3 Haartman Institute, Finland

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Diagnostic Pathology 2013, 8(Suppl 1):S37  doi:10.1186/1746-1596-8-S1-S37

Published: 30 September 2013

First paragraph (this article has no abstract)

Assessment of erythrocytes and leucocytes in thin blood films can be used as an inexpensive diagnostic aid in a series of disease states, e.g. infections, anemia and hematological malignancies. Manual counting of cells is still considered the gold standard for example to establish the level of parasitemia in malaria. However, manual cell counting is time consuming and subject to variability [1]. We here propose an image analysis method that is a combination of adaptive histogram thresholds and morphologic characteristics for the segmentation of red blood cells (RBCs) and white blood cells (WBCs) in digitized thin blood films. The method is implemented on a virtual microscopy platform, the Webmicroscope [2].