Automatic quality analysis of a virtual slide (parameter used: blur) A represents the thumbnail of a whole slide image (H&E staining) whose upper third part is in focus and lower two thirds part is totally out of focus. Each thumbnail B to F shows sharp tiles in green and blurry tiles going from white (a little blurry) to red (the most blurry). Out of 43 tiles at 1.25x (B), 83% were detected as non-blank, and 36% were detected as sharp. For C, D, E and F, the respective values were (146 tiles, 2.5x, 86% non-blank, 34% sharp), (493 tiles, 5.0x, 83% non-blank, 33% sharp), (1751 tiles, 10.0x, 77% non-blank, 31% sharp), (6589 tiles, 20.0x, 76% non-blank, 25% sharp). The WSI is thus considered as of insufficient quality in terms of blurriness, for all its magnification levels being under their respective blur assessment thresholds.
Ameisen et al. Diagnostic Pathology 2013 8(Suppl 1):S23 doi:10.1186/1746-1596-8-S1-S23