The results of the intensity analysis of the digital slide. The DAPI (A), FITC (B) and the rhodamine (C) channels after intensity amplifying. Signals are represented with different intensity in the summarized image (D), therefore an optimal threshold had to be defined in each channel.
Kiszler et al. Diagnostic Pathology 2013 8(Suppl 1):S21 doi:10.1186/1746-1596-8-S1-S21