Figure 3.

Schematic representation of the automated multistep procedure. Image analysis was carried out with the Image-Pro® Plus 5.0 program and data from selected positive objects are exported to an Excel datasheet in which appropriate algorithms are implemented, enabling the definitive number of positive cells to be calculated.

Lejeune et al. Diagnostic Pathology 2013 8(Suppl 1):S13   doi:10.1186/1746-1596-8-S1-S13