Immunohistochemical staining for E-cadherin, β-catenin, and S100A4 in colorectal cancers (×400). E-cadherin, membranous staining at the center of the tumor (A, arrow) and loss or attenuation of expression at the invasive margin of tumor buds (B, arrow). β-catenin, membranous staining (C, arrow) and nuclear staining at the invasive margin of tumor buds (D, arrow). Negative (E, arrow) and positive (F, arrow) staining for S100A4.
Lee et al. Diagnostic Pathology 2013 8:99 doi:10.1186/1746-1596-8-99