Open Access Research

Polymorphism of C3 complement in association with myocardial infarction in a sample of central Tunisia

Nadia Leban1*, Karim Jraba1, Abdelkader Chalghoum1, Selma Hassine1, Donia Elhayek1, Sabri Denden1, Ramzi Lakhdhar1, Faouzi Maatoug2, Habib Gamra2, Hammadi Braham3, Jemni Ben Chibani1 and Amel Haj Khelil1*

Author affiliations

1 Biochemistry and Molecular Biology Laboratory, Faculty of Pharmacy, Street Avicenne, 5019 Monastir, Tunisia

2 Cardiology Department, Fattouma Bourguiba Universitary Hospital, Monastir, Tunisia

3 Biochemistry and Blood Bank Department, Universitary Hospital Taher Sfar, Mahdia, Tunisia

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Citation and License

Diagnostic Pathology 2013, 8:93  doi:10.1186/1746-1596-8-93

Published: 13 June 2013

Abstract

Background

Myocardial infarction (MI) is a major clinical problem because of its large contribution to mortality. The genetic bases of this disease have been widely studied in recent years to find a clear association with some genetic markers that increase the risk of its occurrence. In the present investigation, the correlation between MI and the C3 complement polymorphism was analyzed using a case–control study.

Methods

Our study ported on one hundred seventy survived myocardial infarction patients and ninety five healthy controls. The C3 allele identification was investigated using the amplification refractory mutation system PCR to determine the C3*S and the C3*F alleles of the C3 polymorphism.

Results

Frequencies of C3*S and C3*F in patients are 0.59 and 0.41 respectively. Fisher test results showed a significant increase of C3*F allele in the sample of patients (0.41; odds ratio: 2.616; C.I [1.738-3.938]) compared to controls (0.21; odds ratio: 0.382; 95% CI [0.254-0.575]), p = 2.742 × 10-6.

Conclusion

A strong positive correlation was found between C3 polymorphism and MI estimating that the risk of myocardial infarction is significantly increased among patients with C3*F allele of this polymorphism.

Virtual Slides

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1190484203893646 webcite

Keywords:
Myocardial infarction; C3 complement; Polymorphism; Allele frequency; Association