The positions of the 154240 identified nuclei were obtained from the analysis with ImageJ of the digital slide on a laptop computer. Since the slide was too large to fit into the computer’s memory, it was turned into a mosaic of 16 pieces with overlap of 60 pixels, and each piece underwent automated analysis independently. Then the results were aggregated. The graph shows the probability density function of the distance of a cell nucleus to its nearest neighbor in the whole sample.
Deroulers et al. Diagnostic Pathology 2013 8:92 doi:10.1186/1746-1596-8-92