H&E and HBFP staining, and immunohistochemical staining for S100A1 15 min after ligation. A: The H&E stained sections showed conservation of normal morphology of cardiomyocytes, except for a few cardiomyocytes in the subendocardial layer and papillary muscles, with a weakly eosinophilic cytoplasm (bar: 100 μm). B: Ischemic cardiomyocytes in the subendocardial layer and papillary muscles were positively stained crimson in contrast to yellow for nonischemic cardiomyocytes (HBFP, bar: 100 μm). C: Depletion of S100A1 in a few cardiomyocytes was detected by IHC, which was in agreement with the positively stained areas of HBFP (bar: 100 μm).
Bi et al. Diagnostic Pathology 2013 8:84 doi:10.1186/1746-1596-8-84