Figure 1.

Representative cases of IHC staining and FISH analysis in GC and GEJ adenocarcinoma. A. HER2 IHC staining shows complete, basolateral, or lateral membranous reactivity of strong intensity (3+) in tumor cells (original magnification, ×40). B. HER2 IHC staining shows weak to moderate complete, basolateral, or lateral membranous reactivity (2+) in tumor cells (original magnification, ×100). C. faint/barely perceptible membranous reactivity (1+) in tumor cells (original magnification, ×200). D. no staining (0) in tumor cells (original magnification, ×200). E. a GC case with HER2 amplification using FISH analysis. Green signals refer to the reference probe of Chr. 17 centromere, while red signals are the target probe for HER2. F. A GC case without HER2 amplification using FISH analysis.

Shan et al. Diagnostic Pathology 2013 8:76   doi:10.1186/1746-1596-8-76
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