Cell treatments with DMSO, TNF-α, 17-DMAG, or TNF-α and 17-DMAG together. The normal human bronchial epithelium cell line (NuLi-1) and three lung cancer cell lines (MS-1, A549, and LK-2) were treated with either vehicle control (DMSO), TNF-α (10 ng/ml), 17-DMAG (0.05 μM), or both. Cell counts in each condition were determined by trypan blue exclusion at the time points indicated. (A) NuLi-1 cells; (B) MS-1 cells; (C) A549 cells; (D) LK-2 cells. Cell viability was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrozolium bromide (MTT) assay immediately before (day 0) and after 1, 2, or 3 days of incubation with the drugs. Values are means ± SD for three experiments. It is considered not significant, when P > 0.05 vs. control (DMSO) cell viability of each treatment. *, it is considered as a significant difference, when P < 0.05 vs. corresponding control.
Qu et al. Diagnostic Pathology 2013 8:70 doi:10.1186/1746-1596-8-70