Analysis of GATA4 and DcR1 in glioblastoma; Analysis of GATA4 (A) and DcR1 (B) CpG island promoter methylation status in glioblastoma by the methylation-specific PCR assay. Molecular weight markers are shown in the left, mDNA- methylated DNA control, uDNA-unmethylated DNA control, W- water control, GB1-GB14 glioblastoma tumor samples, NB normal brain sample. The presence of visible PCR products in those lanes marked “U” indicates the presence of unmethylated allele genes and “M” indicates the presence of methylated genes. Kaplan-Meier cumulative survival (months) in glioblastoma patients according to methylation status of (C) GATA4 (log-rank test, p = 0.15) and (D) DcR1 (log-rank test, p = 0.546).
Vaitkienė et al. Diagnostic Pathology 2013 8:7 doi:10.1186/1746-1596-8-7