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Open Access Research

Upregulation of microRNA-224 is associated with aggressive progression and poor prognosis in human cervical cancer

Shu-na Shen1, Ling-feng Wang2, Yong-feng Jia3, Yu-qing Hao4, Lin Zhang1 and Hui Wang1*

Author Affiliations

1 Department of Gynaecology and Obstetrics, The Third Affiliated Hospital of Inner Mongolia Medical University, Baotou 014010, China

2 Department of Burn, The Third Affiliated Hospital of Inner Mongolia Medical University, Baotou 014010, China

3 Pathology Department, The First Affiliated Hospital of Inner Mongolia Medical University, Huhehot 010050, China

4 Dermatological Department, The Third Affiliated Hospital of Inner Mongolia Medical University, Baotou 014010, China

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Diagnostic Pathology 2013, 8:69  doi:10.1186/1746-1596-8-69

Published: 30 April 2013

Abstract

Objective

Accumulating evidence for differential expression of microRNA-224 (miR-224) in various types of human cancer suggests that it may be play a crucial role in tumor biology. The previous microarray detection also shown that miR-224 was one of miRNAs with significant upregulation in cervical cancer tissues relative to adjacent normal tissues. However, little is known about the function of miR-224 in human cervical cancer. The aim of this study was to investigate the clinical significance of miR-224 expression in cervical cancer.

Methods

MiR-224 expression in 126 pairs of fresh human cervical cancer and adjacent normal tissues was measured by real-time quantitative RT-PCR assay.

Results

miR-224 expression was significantly upregulated in cervical cancer tissues when compared with corresponding adjacent normal tissues (P < 0.001). It was also significantly higher in the cancerous tissues of patients with advanced FIGO stage cervical cancer than those with early FIGO stage (P = 0.02). In addition, miR-224 was expressed at significantly higher levels in lymph node metastasis-positive patients than in lymph node metastasis-negative patients (P = 0.008). Moreover, we found that lesser differentiated tumors expressed higher miR-224 (P = 0.03). Finally, there were sufficient evidence to confirm its value in the status of vascular invasion (P = 0.01) and human papillomavirus (HPV) infection (P = 0.02) in cervical cancer. More importantly, Kaplan-Meier analysis showed that cervical cancer patients with high miR-224 expression tend to have shorter overall survival. In multivariate analysis stratified for known prognostic variables, miR-224 was identified as an independent prognostic marker.

Conclusion

Our data indicated that miR-224 upregulation was associated with aggressive progression and poor prognosis in cervical cancer. MiR-224 was identified for the first time as an independent marker for predicting the clinical outcome of cervical cancer patients.

Virtual slides

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/2170449349527493 webcite

Keywords:
MicroRNA-224; Cervical cancer; Real-time quantitative RT-PCR assay; Prognosis