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Open Access Research

Low erythrocyte catalase enzyme activity is correlated with high serum total homocysteine levels in tunisian patients with acute myocardial infarction

Yosri Noichri1*, Abdelkader Chalghoum1, Latifa Chkioua1, Bruno Baudin2, Samia Ernez3, Salima Ferchichi1 and Abdelhédi Miled1

Author affiliations

1 Biochemistry Laboratory CHU Farhat HACHED, Street Doctor Moreau, 4000 Sousse, Tunisia

2 Department of Biochemistry, Hospital Saint-Antoine, 184 Street Faubourg Saint-Antoine, 75571 Paris Cedex 12, France

3 Department of Cardiology, CHU Farhat Hached, Street Doctor Moreau, 4000 Sousse, Tunisia

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Citation and License

Diagnostic Pathology 2013, 8:68  doi:10.1186/1746-1596-8-68

Published: 30 April 2013

Abstract

Background

An imbalance between pro-oxidants and antioxidant systems has been suggested to be implicated in the physiopathology of acute myocardial infarction (AMI). We aimed to evaluate the antioxidant capacity in Tunisian patients and to assess the possible relationship between erythrocyte catalase enzyme activity and hyperhomocysteinaemia.

Methods

108 patients with AMI and 81 healthy subjects were enrolled in this study. Catalase erythrocyte enzyme activity was determined spectrophotometrically whereas “total antioxidant status” (TAS) concentration was measured by a commercially available method. Serum total homocysteine (tHcy) level was determined by a fluorescence polarization immunoassay (FPIA). Lipid peroxidation was measured with a fluorimetric method as “thiobarbituric acid reactive substances” (TBARS).

Results

Compared with healthy subjects, patients with AMI had significantly lower catalase activity (P<0.001), TAS concentrations (P<0.001), and significantly higher serum tHcy (P<0.001) and TBARS levels (P<0.001). Erythrocyte catalase enzyme activity was negatively correlated with serum tHcy and TBARS while serum tHcy and TBARS were in positive correlation. Furthermore, the unbalance between pro-oxidants and antioxidants seems to be more aggravated in patients with Q wave AMI compared to patients with non-Q wave AMI.

Conclusion

Our results suggest the involvement of hyperhomocysteinaemia in the drop of erythrocyte catalase activity related to myocardial ischemia reperfusion. Hyperhomocysteinaemia may increase the myocardial wall dysfunction under ischemia reperfusion by excessive production of reactive oxygen species which is made evident by increased lipid peroxidation.

Virtual slides

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1623509866881834 webcite

Keywords:
Catalase; Hyperhomocysteinaemia; Lipid peroxidation; Acute myocardial infarction