Figure 3.

The expression of B7s and TIM-containing molecules in parapharyngeal liposarcoma sample sections, as detected by immunohistochemistry. (A) Mouse IgG isotype control antibodies showed no positive staining. (B) Goat IgG isotype control antibodies showed no positive staining. (C) B7-H1 was expressed on liposarcoma cell aggregates. (D) B7-DC was expressed on endothelial cells (capillaries). (E) B7-DC was expressed on liposarcoma cell aggregates. (F) B7-H3 was expressed on liposarcoma cell aggregates. (G) B7-H4 was absent from liposarcoma cell aggregates. (H) TIM-1 was absent from liposarcoma cell aggregates. (I) TIM-3 was expressed on liposarcoma cell aggregates. (J) TIM-4 was expressed on liposarcoma cell aggregates. The arrows indicate carcinoma cells, and the arrowheads indicate capillaries. Scale bar = 20 μm.