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Open Access Research

Immunohistochemical detection of Hsp90 and Ki-67 in pterygium

Roberto Sebastiá1*, Marcelo Palis Ventura1, Helena Parente Solari1, Emilia Antecka2, Maria Eugenia Orellana2 and Miguel N Burnier2

Author Affiliations

1 Department of Ophthalmology, Universitary Hospital Federal Fluminense University, Rio de Janeiro, Brazil

2 Henry C. Witelson Ocular Pathology Laboratory, McGill University, Montreal, Canada

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Diagnostic Pathology 2013, 8:32  doi:10.1186/1746-1596-8-32

Published: 21 February 2013

Abstract

Background

To examine the immunohistochemical expression of heat shock protein 90 (Hsp90) and Ki-67 protein in human pterygium.

Materials and methods

Tissues obtained during pterygium surgery of 15 patients who underwent the bare-sclera procedure and 10 normal conjunctivae were studied. All of these pterygia were primary ones. Recurrent pterygia were excluded. Normal bulbar conjunctivas (2 x 2 mm) were obtained from the nasal region close to the limbus from patients during their cataract and retina surgeries. Immunohistochemical detection of Hsp90 and Ki67 was done using the streptavidin–biotin method in paraffin embedded tissue sections.

Results

The percentage of cells stained for Hsp90 was greater for pterygium epithelium (76 ± 10.8) than for normal conjunctiva (1.4 ± 0.8). In each pterigyum sample more than 60% of cells were positive. The differences in positive cells between normal and pterigyum epithelium were highly significant for Hsp90 (P < 0,001).

Pterygium epithelium also showed a higher percentage of cells that stained for Ki67 (10.1 ± 9.5) than for normal conjunctiva (2.1 ± 1.9). The differences in positive cells were also statistically significant for Ki67 (P < 0.01). Although there were significant differences in the majority of samples observed. It was noted that in some samples there was no difference between normal and pterygium epithelium for Ki67.

Conclusion

Our results indicate an abnormal expression of Hsp90 and ki-67 in pterygium samples when compared to normal conjunctiva.The finding of abnormal expression of levels of Hsp90 in pterygium samples can stimulate new research into pterygium and its recurrence.

Virtual Slides

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1128478792898812 webcite

Keywords:
Pterygium; Immunohistochemistry; Hsp90; Ki-67