Judgment for p16 immunohistochemistry (IHC) and In situ hybridization (ISH) for high- risk HPV-DNA. (A) A representative p16 IHC-positive case, showing intense nuclear and cytoplasmic staining for more than 70% of tumor cells. (B) A representative ISH positive case for high-risk HPV-DNA, showing hybridization signals visualized as punctate dots. [(A) IHC for p16, original magnification, x200, (B) ISH for HPV-DNA, original magnification, x300].
Fujimaki et al. Diagnostic Pathology 2013 8:211 doi:10.1186/1746-1596-8-211