An algorithm for the judgment of high-risk HPV association with oropharyngeal carcinoma. Cases positive for both p16 IHC and ISH were judged as high-risk HPV-associated oropharyngeal carcinoma (HPV-OPC). ISH specific for type 16/18 HPV was conducted for all cases. Cases discordantly p16 positive by IHC but HPV16/18 negative by ISH were further evaluated for wide-spectrum HPV types (types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 68). Number of cases corresponding to each is shown in parentheses.
Fujimaki et al. Diagnostic Pathology 2013 8:211 doi:10.1186/1746-1596-8-211