PRMT5 overexpression in lung cancer is evident at mRNA and protein levels. (a) There is a 6.13-fold increase in PRMT5 mRNA levels in lung tumors (LT), 8 cases, over matched nonneoplastic pulmonary parenchyma (L), as evident by TaqMan real time polymerase chain reaction (PCR). (b) PRMT5 and its symmetric methylation mark H4R3 are detected in lung carcinoma cell lines (NCI-A549, NCI-H520) but not in the nonneoplastic human pulmonary alveolar (HPAEpiC) and bronchial epithelial cell lines (HBEpiC); cellular localization experiments highlight nuclear and cytoplasmic fractions of PRMT5; Western immunoblot (b, c); immunohistochemistry (d), NCI-H69, original magnification ×600.
Shilo et al. Diagnostic Pathology 2013 8:201 doi:10.1186/1746-1596-8-201