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Open Access Research

The association between chemosensitivity and Pgp, GST-π and Topo II expression in gastric cancer

Ming Geng1, Lin Wang2, Xin Chen1, Ruixue Cao1 and Peifeng Li1*

Author Affiliations

1 Department of Pathology, General Hospital of Jinan Military Command, Jinan, China

2 Department of Laboratory Diagnosis, General Hospital of Jinan Military Command, Jinan, China

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Diagnostic Pathology 2013, 8:198  doi:10.1186/1746-1596-8-198

Published: 10 December 2013

Abstract

Background

To investigate the relationship between P-glycoprotein (Pgp), glutathione S-transferase π (GST-π) and topoisomerase II (Topo II) expression and human gastric cancer chemoresistance in vitro.

Methods

Primary single-cell suspensions were prepared from fresh specimens of primary gastric cancer and exposed to hydroxycamptothecin (HCPT), cisplatin (CDDP), 5-fluorouracil (5-FU), adriamycin (ADM) and mitomycin (MMC) for 48 h. Cell metabolic activity and rate of inhibition were evaluated using tetrazolium (MTT) assay. Pgp, GST-π and Topo II expression was determined in gastric carcinoma tissue samples using immunohistochemistry.

Results

Chemosensitivity of the gastric cancer cells varied; the rates of inhibition of cells exposed to HCPT, CDDP and 5-FU were significantly higher than that of cells exposed to ADM and MMC (p < 0.05). Gastric cancer cells with Pgp expression were resistant to ADM and HCPT (p = 0.008 and p = 0.011, respectively). Cells resistant to 5-FU, CDDP and MMC had significantly higher GST-π expression (p < 0.05). Topo II expression was significantly lower in cells resistant to HCPT, ADM and MMC (p < 0.05). Pgp and GST-π expression may contribute to primary resistance of gastric cancer cells to some chemotherapeutic drugs, while Topo II expression may indicate HCPT, ADM and MMC sensitivity.

Conclusions

Pgp, GST-π and Topo II detection and the MTT assay could be used as predictors in chemotherapeutic drug administration and for identifying drug resistance in gastric carcinoma.

Virtual slides

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/3448329111142964 webcite.

Keywords:
Gastric cancer; MTT colorimetric assay; Drug sensitivity; Pgp; GST-π; Topo II