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Association of a polymorphism in PON-1 gene with steroid-induced osteonecrosis of femoral head in Chinese Han population

Zhiyao Wang1, Yanqiong Zhang2, Xiangying Kong2, Shangzhu Li3, Yimin Hu3, Rongtian Wang1, Yan Li1, Chao Lu1, Na Lin2* and Weiheng Chen1*

Author Affiliations

1 Wangjing hospital (Hospital of Orthopedics and Traumatology), China Academy of Chinese Medical Sciences, Beijing 100102, China

2 Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China

3 Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300041, China

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Diagnostic Pathology 2013, 8:186  doi:10.1186/1746-1596-8-186

Published: 8 November 2013



Treatment with steroids covers a wide spectrum of diseases in clinic. However, some users are suffering from serious side effects of steroid administration, while we enjoy the benefit it brings about. Osteonecrosis of the femoral head (ONFH) is a troublesome one among them. Recent studies have demonstrated that lipid metabolism disorder may play a vital role in pathogenesis of ONFH and mutation of the paraoxonase-1 (PON-1) gene may be involved in the occurrence of this disease. However, the relationship between polymorphisms of PON-1 and ONFH has not been thoroughly studied. The aim of this study was to determine whether PON-1 polymorphisms are associated with steroid-induced ONFH through a cohort study among Chinese Han population.


This trial applied a case–control scheme to compare the clinical data including PON-1 SNP among 94 patients and 106 control subjects to analyze the association between SNP and risk of steroid-induced ONFH. Time of Flight Mass Spectrometer is utilized for genotyping and the result was analyzed in multivariate analysis models.


According to polymorphism test of rs662, its SNP was significantly associated with the risk of ONFH in overdominant analysis model [P value: 0.022; odds ratio (OR): 0.39]. However, genotype frequencies of rs662 of PON-1 gene between case and control group showed no differences (P > 0.05).


Our data suggest for the first time that SNP (rs662) of the PON-1 gene was associated with the risk of steroid-induced ONFH. In addition, PAI-1 SNPs may play an important role in pathogenesis of ONFH.

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Steroid-induced osteonecrosis of the femoral head; Paraoxonase-1; Single nucleotide polymorphism