Email updates

Keep up to date with the latest news and content from Diagnostic Pathology and BioMed Central.

Open Access Highly Accessed Research

Astragalus saponins affect proliferation, invasion and apoptosis of gastric cancer BGC-823 cells

Tao Wang1, Xiaoyan Xuan2, Min Li2, Ping Gao1, Yuling Zheng3, Wenqiao Zang2* and Guoqiang Zhao2*

Author Affiliations

1 Department of Hemato-tumor, The First Affiliated Hospital of Henan University of TCM, Zhengzhou, People’s Republic of China

2 Department of Microbiology and Immunology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, People’s Republic of China

3 Henan University of TCM, Zhengzhou, People’s Republic of China

For all author emails, please log on.

Diagnostic Pathology 2013, 8:179  doi:10.1186/1746-1596-8-179

Published: 24 October 2013

Abstract

Background

Astragalus memebranaceus is a traditional Chinese herbal medicine used in treatment of common cold, diarrhea, fatigue, anorexia and cardiac diseases. Recently, there are growing evidences that Astragalus extract may be a potential anti-tumorigenic agent. Some research showed that the total saponins obtained from Astragalus membranaceus possess significant antitumorigenic activity. Gastric cancer is one of the most frequent cancers in the world, almost two-thirds of gastric cancer cases and deaths occur in less developed regions. But the effect of Astragalus membranaceus on proliferation, invasion and apoptosis of gastric cancer BGC-823 cells remains unclear.

Methods

Astragalus saponins were extracted. Cells proliferation was determined by CCK-8 assay. Cell cycle and apoptosis were detected by the flow cytometry. Boyden chamber was used to evaluate the invasion and metastasis capabilities of BGC-823 cells. Tumor growth was assessed by subcutaneous inoculation of cells into BALB/c nude mice.

Results

The results demonstrated that total Astragalus saponins could inhibit human gastric cancer cell growth both in vitro and in vivo, in additional, Astragalus saponins deceased the invasion ability and induced the apoptosis of gastric cancer BGC-823 cells.

Conclusions

Total Astragalus saponins inhibited human gastric cancer cell growth, decreased the invasion ability and induced the apoptosis. This suggested the possibility of further developing Astragalus as an alternative treatment option, or perhaps using it as adjuvant chemotherapeutic agent in gastric cancer therapy.

Keywords:
Gastric cancer; Proliferation; Invasion; Apoptosis