Table 2

Antibodies used for immunohistochemistry
Antibody Pretreatment Incubation Detection Dilution Company
AMACR Monoclonal 98°C Hot water, pH 9.0, 15 min Envision™ Ready-to-Use Kit Dako, Glostrup, Denmark
p16Ink Monoclonal, clone G175-405 98°C Hot water, pH 6.1, 20 min Envision™ 1:10 BD Biosciences, Heidelberg, Germany
Ki67 Monclonal, clone MIB-1 98°C Hot water, pH 9.0, 15 min Envision™ Ready-to-Use Kit Dako, Glostrup, Denmark
BRAF Monoclonal, clone VE1 37°C Hot water, PH 9.0 30 min Envision™ 1:100 DCS Innovative Diagnostik, Hamburg, Germany
MLH1 Monoclonal, clone ES05 98°C, pH 9.0 20 min Envision™ Ready-to-Use Kit Dako, Glostrup, Denmark
Beta-catenin Monoclonal 37°C water bath 30 min 20 min Zytochem Plus HRP Polymer (Zytomed Systems, Berlin, Germany) 1:2000 BD Biosciences, Heidelberg, Germany
CK20 Monoclonal, Clone Ks20.8 98°C, pH 9.0 30 min Envision™ Ready-to-Use Kit Dako, Glostrup, Denmark

Dayi et al.

Dayi et al. Diagnostic Pathology 2013 8:178   doi:10.1186/1746-1596-8-178

Open Data