Taqman qRT-PCR detection of relative miR-497 expression in tissue samples. A. qRT-PCR was performed to respectively detect the relative miR-497 expression in 30 pairs of BC and corresponding noncancerous breast tissues. B. The mean expression level of miR-497 in BC tissues was significantly lower than that in corresponding noncancerous breast tissues (P = 0.0046). U6 snRNA was used as an internal control. Each assay was performed at least in triplicate. Corresponding P values analyzed by t-tests are indicated. T: BC tissues; N: noncancerous breast tissues.
Wang et al. Diagnostic Pathology 2013 8:172 doi:10.1186/1746-1596-8-172