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Open Access Research

Expression of CD44v6 and integrin-β1 for the prognosis evaluation of pancreatic cancer patients after cryosurgery

Gang Zhou12, David Chiu12, Dajiang Qin1, Lizhi Niu12, Jinlei Cai1, Lihua He12, Dongfeng Tan3 and Kecheng Xu12*

Author affiliations

1 Department of Oncology, The GIBH Affiliated Fuda Hospital, Chinese Academy of Sciences, Guangzhou 510305, China

2 Department of Oncology, Fuda Cancer Hospital, Guangzhou 510665, China

3 Department of Pathology, MD Anderson Cancer Center, TX 77030, USA

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Citation and License

Diagnostic Pathology 2013, 8:146  doi:10.1186/1746-1596-8-146

Published: 2 September 2013

Abstract

Background

Many previous studies demonstrated that cell adhesion molecules CD44v6 and integrin-β1 had been extensively investigated as potential prognostic markers of various cancers. However, data in PC are scarce.

Methods

We now investigate CD44v6 and integrin-β1 mRNA expression in PBMC by a triplex real-time RT-PCR assay and protein expression in plasma by ELISA. All specimens were collected from 54 PC patients who received the treatment of cryosurgery as well as 20 healthy individuals (control).

Results

The mRNA and protein expression levels of CD44v6 and integrin-β1 in patients were significantly increased compared with control group (P<0.05). The high CD44v6 mRNA and protein expression were significantly correlated with clinical stage, tumor differentiation, LNM, liver metastasis and decreased median DFS (P<0.05), while the high integrin-β1 mRNA and protein expression were significantly correlated with clinical stage, LNM, liver metastasis and decreased median DFS (P<0.05). Clinical stage, LNM, liver metastasis, CD44v6 mRNA and protein expression were the independent predictors of survival in PC patients (P<0.05). Moreover, CD44v6 and integrin-β1 mRNA and protein expression levels were significantly decreased in patients in 3 months after cryosurgery (P<0.05). No significant difference was found in CD44v6 mRNA and protein expression between patients in 3 months after cryosurgery and control group (P>0.05).

Conclusion

CD44v6 and integrin-β1 mRNA and protein expression in blood may serve as biomarkers for the development and metastasis of PC, and as prognostic indicators for PC. They may become useful predictors in assessing outcome of PC patients after cryosurgery.

Virtual slides

The virtual slides for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/4035308681009006. webcite

Keywords:
PC; CD44v6; Integrin-β1; mRNA; Protein; Cryosurgery