Punch biopsy from the scalp with malignant melanoma. 3a and 3b: Hemotoxylin and Eosin stain (20× and 100×). There is an atypical melanocytic proliferation with a predominant dermal component, but with individual atypical junctional nests seen. 3c and 3d: Hemotoxylin and Eosin stain (200× and 400×). The atypical melanocytes are predominantly epithelioid with light eosinophilic cytoplasm, hyperchromatic and vesicular nuclei with variable prominent nucleoli. Scattered mitotic figures were seen, including in the deeper portion of the lesion. 3e and 3f: Melan-A immunostain (20× and 100×).
Gru and Lu Diagnostic Pathology 2013 8:135 doi:10.1186/1746-1596-8-135