Email updates

Keep up to date with the latest news and content from Diagnostic Pathology and BioMed Central.

Open Access Research

Serum peptidome patterns of hepatocellular carcinoma based on magnetic bead separation and mass spectrometry analysis

Xia Ying, Su-xia Han, Jun-lan Wang, Xia Zhou, Gui-hua Jin, Long Jin, Hao Wang, Lei Wu, Jianying Zhang and Qing Zhu*

Author affiliations

Department of Medical Oncology, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shannxi, PR China

For all author emails, please log on.

Citation and License

Diagnostic Pathology 2013, 8:130  doi:10.1186/1746-1596-8-130

Published: 5 August 2013

Abstract

Background

Hepatocellular carcinoma (HCC) is one of the most common cancers in the world,and the identification of biomarkers for the early detection is a relevant target. The purpose of the study is to discover specific low molecular weight (LMW) serum peptidome biomarkers and establish a diagnostic pattern for HCC.

Methods

We undertook this pilot study using a combined application of magnetic beads with Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique and ClinPro Tools v2.2 to detect 32 patients with HCC, 16 patients with chronic hepatitis (CH), 16 patients with liver cirrhosis (LC) and 16 healthy volunteers.

Results

The results showed 49, 33 and 37 differential peptide peaks respectively appeared in HCC, LC and CH groups. A Supervised Neural Network (SNN) algorithm was used to set up the classification model. Eleven of the identified peaks at m/z 5247.62, 7637.05, 1450.87, 4054.21, 1073.37, 3883.64, 5064.37, 4644.96, 5805.51, 1866.47 and 6579.6 were used to construct the peptides patterns. According to the model, we could clearly distinguish between HCC patients and healthy controls as well as between LC or CH patients and healthy controls.

Conclusions

The study demonstrated that a combined application of magnetic beads with MALDI-TOF MB technique was suitable for identification of potential serum biomarkers for HCC and it is a promising way to establish a diagnostic pattern.

Virtual slides

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1503629821958720 webcite.

Keywords:
Hepatocellular carcinoma; Chronic hepatitis; Liver cirrhosis; Magnetic beads; Matri-assisted laser desorption/ionization time-of-flight mass spectrometry; Serum biomarkers