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Insights into the prognostic value of DJ-1 and MIB-1 in astrocytic tumors

Rasha M Abd El Atti1*, Hoda H Abou Gabal1, Wesam M Osman1 and Amr S Saad2

Author Affiliations

1 Department of Pathology, Faculty of Medicine, Ain Shams University, Cairo, Egypt

2 Clinical Oncology, Faculty of Medicine, Ain Shams University, Cairo, Egypt

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Diagnostic Pathology 2013, 8:126  doi:10.1186/1746-1596-8-126

Published: 31 July 2013

Abstract

Background

The histological grade is the gold standard for the evaluation of prognosis of astrocytic tumors. Nevertheless, morphologic criteria are not always accurate prognostic indicators.

Aim

The research investigates the expression of MIB-1 and DJ-1 in different grades of astrocytomas and evaluates the possible prognostic role of DJ-1 in these tumors in relation to other prognostic parameters including the MIB-1 labeling index.

Materials and methods

Immunohistochemical expression of MIB-1 and DJ-1 was evaluated in 111 samples of astrocytic tumors comprising 28 diffuse astrocytomas, 38 anaplastic astrocytomas and 45 glioblastomas. The univariate survival analysis was done using the Kaplan-Meier method and the multivariate survival analysis was done using Cox proportional hazard model.

Results

The statistical analysis revealed a significant correlation between each of DJ-1 and MIB-1 and the histological grade of astrocytomas. The univariate analysis showed that high grade, high DJ-1 score and MIB-1 labeling index ≥ 10.1 were associated with poor survival. Multivariate analysis for all the studied astrocytomas proved the independent prognostic significance of the histological grade and DJ-1 score. Meanwhile, the multivariate analysis for each grade emphasized that DJ-1 was the only independent prognostic indicator in high-grade astrocytomas.

Conclusion

This study emphasized the effectiveness of high DJ-1 expression in predicting poor survival of astrocytoma patients, when compared to MIB-1. DJ-1 could be particularly important in cases with discrepancies between the morphologic criteria and clinical parameters.

Virtual slides

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1070116023943146 webcite

Keywords:
DJ-1; MIB-1; Immunohistochemistry; Prognosis; Astrocytoma