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Histopathological and clinical evaluation of Kombucha tea and Nitrofurazone on cutaneous full-thickness wounds healing in rats: an experimental study

Fardin Barati1, Javad Javanbakht2*, Farajollah Adib-Hashemi1, Ehsan Hosseini3, Reyhaneh Safaeie4, Mojtaba Rajabian5, Mostafa Razmjoo6, Reza Sedaghat7 and Mehdi Aghamohammad Hassan1

Author affiliations

1 Department of Clinical Science, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran

2 Department of Pathology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran

3 Faculty of Para Veterinary Medicine, Ilam University, Ilam, Iran

4 Gradaute, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran

5 Food Hygiene Department, University of Shahekord, Shahekord, Iran

6 Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

7 Department of Pathology, Faculty of Medicine Science, Shahed University, Tehran, Iran

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Citation and License

Diagnostic Pathology 2013, 8:120  doi:10.1186/1746-1596-8-120

Published: 17 July 2013



Kombucha, a fermented tea (KT) is claimed to possess many beneficial properties. The aim of this study was to evaluate clinical and histopathological alterations of Kombucha tea and Nitrofurazone on cutaneous full-thickness wounds healing in rat.


In present study 24 Wister -albino rats weighing 150–200 g were selected and divided to two treatment groups as Nitrofurazone ointment (0.2%) and Kombucha tea. Subsequently, the anesthesia was exerted by Ketamin hydrochloride 10% (40 mg/kg) and Xylasine (2 mg/kg) through intra muscular (IM) route. Furthermore, upon preparation of dorsal region of the animal for surgery, a piece of full-thickness skin removed (2 × 2 cm). In order to comparing wounds healing clinically and histologically, once every four days from the commencement, the wounds were photographed and the healed surface was measured by Scion image software.


The clinical findings indicated that the Kombucha fungus resulted in precipitating healing than Nitrofurazone; however, it was not significant (p > 0.05). In order to pathological comparing of wound healing process, several wound biopsies were taken on 4, 8, 12, 16 and 20th days. Additionally, the histopathological results demonstrated that there was inflammation in Nitrofurazone group through twelveth day, somehow the epithelium was formed and abundant vessels were visible. Although on 16th day and the previous days the healing condition of Kombucha fungus was considered as minimal rate, revealing it is similar to Nitrofurazone group on 20th day.


To wrap up. These observations suggest that the Kombucha fungus healing quality was rapid from 12th day to the end of the research, whereas no significant difference was observed.

Virtual slide

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Wound healing; Kombucha fungus; Nitrofurazone; Histopathological; Clinical