Results of the immunohistochemical examination of high-grade carcinoma component in the present case. Typical AdCC area showed low labeling index for Ki-67, whereas the high-grade carcinoma component (asterisk) showed a high Ki-67 labeling index (A: immunostaining x20). The high-grade carcinoma component also showed strong and diffuse positivity for p53 (B: immunostaining x200: inset; weakly positive for p53 in typical AdCC), Her-2 (C; inset; weakly positive for Her-2 in typical AdCC: immunostaining x400), p16 (D: immunostaining x200: inset; focally positivity for p16 in typical AdCC; immunostaining x400), and cyclin A (E; immunostaining x200: inset; focally positivity for cyclin A in typical AdCC; immunostaining x400), respectively.
Kusafuka et al. Diagnostic Pathology 2013 8:113 doi:10.1186/1746-1596-8-113