Table 2

Associations of MSI screening status and beta-catenin expression with expression of p21, p27 and p53
MSI screening status Beta-catenin score
n (%) MSS MSI P-value 0-1 2-3 4-5 P-value
438(85.0) 77(15.0) 159(30.2) 170(32.3) 198(37.6)
P53 status
 Negative 196(40.0) 62(80.5) <0.001** 101(65.2) 92(55.1) 76(38.6) <0.001**
 Positive 230(54.0) 15(19.5) 54(34.8) 75(44.9) 121(61.4)
 missing 12 0 4 3 1
Cyclin D1 fraction
 0-1% 94(22.1) 2(2.6) <0.001** 36(23.4) 36(21.7) 31(15.8) 0.001**
 2-25% 159(37.4) 26(34.2) 63(40.9) 65(39.2) 63(32.1)
 26-50% 65(15.3) 16(21.1) 20(13.0) 29(17.5) 33(16.8)
 51-75% 87(20.5) 25(32.9) 27(17.5) 32(19.3) 53(27.0)
 >75% 20(4.7) 7(9.2) 8(5.2) 4(2.4) 16(8.2)
 missing 13 1 5 4 2
Cyclin D1 intensity
 Negative 94(22.1) 2(2.6) <0.001** 36(23.4) 36(21.7) 31(15.8) 0.085
 Weak 154(36.2) 20(26.3) 51(33.1) 59(35.5) 68(34.7)
 Moderate 129(30.4) 41(53.9) 49(31.8) 55(33.1) 68(34.7)
 Strong 48(11.3) 13(17.1) 18(11.7) 16(9.6) 29(14.8)
 missing 13 1 5 4 2
P27 fraction
 0-1% 54(12.7) 24(31.2) 0.003** 43(27.9) 33(19.8) 8(4.1) <0.001**
 2-25% 96(22.6) 14(18.2) 36(23.4) 43(25.7) 35(17.9)
 26-50% 90(21.2) 16(20.8) 32(20.8) 40(24.0) 36(18.4)
 51-75% 134(31.5) 15(19.5) 31(20.1) 36(21.6) 83(42.3)
 >75% 51(12.0) 8(10.4) 12(7.8) 15(9.0) 34(17.3)
 missing 13 1 5 3 2
P27 intensity
 Negative 54(12.7) 24(31.2) <0.001** 43/27.9) 33(19.8) 8(4.1) <0.001**
 Weak 96(22.6) 22(28.6) 46(29.9) 39(23.4) 37(18.9)
 Moderate 142(33.4) 18(23.4) 50(32.5) 51(30.5) 62(31.6)
 Strong 133(31.3) 13(16.9) 15(9.7) 44(26.3) 89(45.4)
 missing 13 1 5 3 2
P21 fraction
 0-1% 69(16.3) 3(3.9) 0.002** 25(16.2) 27(16.2) 25(12.9) 0.835
 2-25% 233(55.1) 41(53.2) 81(52.6) 90(53.9) 112857.7)
 26-50% 60(14.2) 18(23.4) 24(15.6) 28(16.8) 26(13.4)
 51-75% 53(12.5) 13(16.9) 21(13.6) 16(9.6) 30(15.5)
 >75% 8(1.9) 2(2.6) 3(1.9) 6(3.6) 1(0.5=
 missing 15 1 5 3 4
P21 intensity
 Negative 69(16.3) 3(3.9) <0.001** 25(16.2) 27(16.2) 25(12.9) 0.567
 Weak 174(41.1) 24(31.2) 60(39.0) 63(37.7) 81(41.8)
 Moderate 122(28.8) 36(46.8) 51(33.1) 52(31.1) 60(30.9)
 Strong 58(13.7) 14(18.2) 18(11.7) 25(15.0) 28(14.4)
 missing 15 1 5 3 4
MSI screening status
 MSS - - 109(72.7) 136(83.4) 184(95.8) <0.001**
 MSI - - 41(27.3) 27(16.6) 8(4.2)
 missing 9 7 6

Columnar percentage in parentheses. χ2-test of association was used for 2x2 tables and χ2-test for linear trend for tables with more than two rows and/or columns. The categories marked as missing were not included in the analysis. *Significant at the 0.05 level. **Significant at the 0.01 level. MSS = Microsatellite stable, MSI = Microsatellite unstable.

Wangefjord et al.

Wangefjord et al. Diagnostic Pathology 2013 8:10   doi:10.1186/1746-1596-8-10

Open Data