Table 2 |
||||
| Applied antibodies and conditions for primary immunohistochemical reaction | ||||
| N-Cadherin | E-Cadherin | P-Cadherin | KSP-Cadherin | |
| Clone | 6 G11 (mouse) | NCH-38 (mouse) | 56C1 (mouse) | MRQ-33 (mouse) |
| Source | Dako, Hamburg | Dako, Hamburg | Linaris, Dossenheim | Zytomed Systems, Berlin |
| Germany | Germany | Germany | Germany | |
| Pretreatment | citrate buffer | citrate buffer | citrate buffer | citrate buffer |
| pH6.0 40 min | pH6.0 40 min | pH6.0 40 min | pH6.0 40 min | |
| Dilution and | 1:50 30 min RT | 1:50 30 min RT | 1:100 30 min RT | 1:50 30 min RT |
| Incubation | ||||
RT: room temperature.
Behnes et al. Diagnostic Pathology 2012 7:95 doi:10.1186/1746-1596-7-95
