Figure 4 .
Representative examples of MS-PCR analysis of 82 induced sputum clinical samples. Bisulfite-modified DNA was amplified with specific primers to detect methylated DNA (M) and unmethylated DNA (U); MW: Molecular weight marker; in vitro genomic DNA treated with SssI enzyme used as positive control for methylated genes. (CM); peripheral blood lymphocytes DNA used as a control of unmethylated genes (CU); H20: distilled water used as negative control in the PCR amplification.
Guzmán et al. Diagnostic Pathology 2012 7:87 doi:10.1186/1746-1596-7-87