Figure 4 .

Representative examples of MS-PCR analysis of 82 induced sputum clinical samples. Bisulfite-modified DNA was amplified with specific primers to detect methylated DNA (M) and unmethylated DNA (U); MW: Molecular weight marker; in vitro genomic DNA treated with SssI enzyme used as positive control for methylated genes. (CM); peripheral blood lymphocytes DNA used as a control of unmethylated genes (CU); H₂0: distilled water used as negative control in the PCR amplification.