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Oncogenic reg IV is a novel prognostic marker for glioma patient survival

Qi Wang1, Jianping Deng2, Jun Yuan3, Liang Wang2, Zhenwei Zhao2, Shiming He2, Yongsheng Zhang3* and Yanyang Tu1*

Author Affiliations

1 Department of Experimental Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an City, 710032, China

2 Department of Neurosurgery, Tangdu Hospital, Fourth Military Medical University, Xi'an City, 710032, China

3 Department of Administrative, Tangdu Hospital, Fourth Military Medical University, Xi'an City, 710032, China

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Diagnostic Pathology 2012, 7:69  doi:10.1186/1746-1596-7-69

Published: 19 June 2012



The aberrant expression of regenerating islet-derived family member, 4 (Reg IV) has been found in various human cancers. However, the roles of Reg IV gene and its encoding product in human glioma have not been clearly understood. Therefore, the aim of this study was to investigate the clinicopathological significance of Reg IV expression in glioma.


Reg IV mRNA and protein expression in human gliomas and non-neoplastic brain tissues were respectively detected by real-time quantitative RT-PCR assay, Western blot, and immunohistochemistry. The association of Reg IV immunostaining with clinicopathological factors and prognosis of glioma patients was also statistically analyzed.


Reg IV mRNA and protein expression levels in glioma tissues were both significantly higher than those in the corresponding non-neoplastic brain tissues (both P < 0.001). Additionally, the increased Reg IV immunostaining in glioma tissues was significantly associated with advanced pathological grade (P = 0.008). Reg IV protein up-regulation was also significantly correlated with low Karnofsky performance score (KPS) (P = 0.02). Moreover, the overall survival of patients with high Reg IV protein expression was dramatically shorter than those with low Reg IV protein expression (P < 0.001). Multivariate Cox regression analysis further confirmed that Reg IV expression was an independent prognostic factor for patients with gliomas (P = 0.008).


These convinced evidences suggest for the first time that Reg IV might accelerate disease progression and act as a candidate prognostic marker for gliomas.

Virtual slides

The virtual slide(s) for this article can be found here: webcite

Regenerating islet-derived family member 4; glioma; RT-PCR; Western blot; Immunohistochemistry; Prognosis