Comparisons between cavitronic ultrasonic surgical aspiration (CUSA) and FFPE specimens for all normalized CN ratios. A. Bland-Altman analysis demonstrated that normalized CN ratios ([tumor DNA]/[NB DNA]) obtained from either source of DNA corresponded significantly (within 1.96 SD limits, dotted lines) for oncogene amplifications up to at least 15-fold (log value of 1.18). The logarithmic scale was used on the x-axis to separate ratio values for the majority of the data points as much as possible. Note that a normal CN ratio of 1 is equivalent to a log value of 0. The CN ratios for all genes tested with each kit (multiple probes) in all tumors were included, n = 889. B. The CN ratios derived from the two sources of DNA exhibited strong correlations in each tumor with results from each kit shown separately. Assays using the MLPA kits, P171, P172, and P173, had n’s equal to 42, 42 and 43, respectively.
Truong et al. Diagnostic Pathology 2012 7:66 doi:10.1186/1746-1596-7-66