Table 1 |
||||
| Nucleotide primer sequences used for real-time quantitative PCR | ||||
| Target genes | Nucleotide sequence | Orientation | Amplicon length | Accession No. |
| Bak |
5′— CCCAGGACACAGAGGAGGTTT—3′ 5′— GCCTCCTGTTCCTGCTGATG—3′ |
Sense Antisense |
65 bp | NM001188 |
| Bcl-xl |
5′— TGCGTGGAAAGCGTAGACAA—3′ 5′—ATTCAGGTAAGTGGCCATCCAA—3′ |
Sense Antisense |
75 bp | NM138578 |
| Mcl-1 |
5′— AGGCTGGGATGGGTTTGTG—3′ 5′—CACATTCCTGATGCCACCTTCT—3′ |
Sense Antisense |
65 bp | NM021960 |
| GAPDH |
5′- AACAGCCTCAAGATCATCAGCA-3′ 5′- CATGAGTCCTTCCACGATACCA-3′ |
Sense Antisense |
102 bp | M33197 |
Zhang et al. Diagnostic Pathology 2012 7:65 doi:10.1186/1746-1596-7-65
